Low Binding Beads
Cell Based Assays
of Protein Stability
OPS Diagnostics is excited to offer three
new and novel products that help simplify freeze drying.
These products are pre-formulated
excipients/lyoprotectants provided as sterile liquid
reagents. The Microbial Freeze Drying Buffer is a BSA-free
formulation with properties far superior to sucrose and
skim milk. The protein Lyophilization Reagent (2X
Concentrate) is formulated for protein stability and
provides superb caking properties with properties
exceeding other protein stabilizers. The Freeze Drying Indicator
solution is based on the 2X Concentrate but with an added
indicator that turns color upon drying. The indicator is
an excellent tool for monitoring the lyophilization
process, for mapping freeze drying within a chamber, and
for assessing the freeze drying process within individual
Freeze Drying Indicator in action.
The blue sample is dry while the
pink sample contains more than 2% moisture.
Lyophilization Reagent (2X
This clear solution is designed to simplify protein
lyophilization. It is composed of a mixture of a
matrix (caking agent) and lyoprotectant, that when mixed
act synergistically to provide a solution with a high
eutectic point, solid matrix, and excellent protein
stability. It is prepared as a 2X concentrate that can be
used as is, or mixed in equal concentration with a
sample. Mixing the 2X Concentrate with buffers may
require a reassessment of freeze drying parameters.
Lyophilization Reagent is sterile filtered and bottled aseptically.
It is provided in 100 ml and 500 ml PET bottles.
Freeze Drying Indicator:
500 ml ($58)
This colorimetric indicator reagent changes
color during the freeze drying process giving the user a
real-time measurement of dryness. This formulation
combines the protein stabilizers with an
indicator dye. It can be used in parallel with the
standard reagent to show when freeze drying is completed.
This indicator reagent should be run along side of
critical samples and not used as the lyoprotectant itself
(protein lyophilization with the indicator should be
discarded). This reagent is also useful for mapping and
validating freeze dryers. The reagent can be used as a
visual tool for operational qualification of large and
small freeze dryer units. It is filter sterilized and
aseptically bottled in 100 ml and 500 ml PET bottles.
Freeze Drying Buffer: 500
The Microbial Freeze Drying Buffer
is evolved from a standard solution described in the ATCC
lyophilization manual. To use, cultured
microorganisms are mixed with the buffer and simply freeze
dried. Viability of bacteria in this buffer is over
90%, far exceeding methods relying on sucrose and skim
milk. This product is sterile filtered and
aseptically filled in 100
ml and 500 ml PET bottles.